Peptide Stability Testing Methods: How Researchers Measure Degradation
From accelerated stress testing to long-term shelf-life programs, here are the techniques researchers use to quantify peptide stability.
Stability testing answers a simple question with a complicated answer: how long is this peptide actually good for, and under what conditions?
Forced degradation studies
Researchers deliberately expose samples to elevated temperature, oxidative stress (hydrogen peroxide), acid/base hydrolysis, and light. The resulting degradation profile reveals which pathways the molecule is most vulnerable to.
RP-HPLC and mass spec monitoring
Reverse-phase HPLC tracks the appearance of new peaks over time. Coupled with mass spectrometry, researchers can identify whether new peaks are oxidation products, truncated sequences, or aggregates.
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